- 25 ml ASTM Volumetric Flask
- Includes solid glass No. 9 stopper
- Available with blue or Amber printing
- Class A
- Tolerance ±0.030 ml
- Made from high-quality 3.3 borosilicate glass
- Low temperature gradient – can withstand high temperatures and thermal shocks*
- Superior chemical durability*
- Autoclavable
- Complies with ASTM-E288
OVERVIEW:
GWSI 25 ml ASTM Class A volumetric flasks with glass stoppers provide specialized micro-volume precision for preparing ultra-concentrated stock solutions from extremely precious or rare materials. Made from borosilicate 3.3 glass, they deliver excellent chemical inertness and thermal stability while maximizing the utility of compounds available in sub-milligram quantities. Each flask is NABL calibrated to meet stringent ASTM Class A tolerances, offering reliable accuracy for advanced research applications where every microgram of material is critically valuable.
HISTORY:
The 25 ml volumetric flask emerged as specialized analytical techniques required working with extremely limited sample quantities, often isolated natural products or newly synthesized pharmaceutical compounds available only in microgram amounts. This minimal capacity allowed researchers to prepare precise stock solutions from precious materials that would be impossible to obtain in larger quantities. The size became essential in drug discovery, natural product chemistry, and advanced materials research where compounds are synthesized or isolated in milligram or sub-milligram batches, making precise micro-volume preparation absolutely critical.
APPLICATIONS;
– Pharmaceutical labs — preparing ultra-concentrated stocks from newly synthesized drug candidates available in microgram quantities, specialized reference standards
– Biotech facilities— concentrated standards from rare, isolated compounds, reagent preparation with precious biological materials available in limited amounts
– **Clinical laboratories — preparing ultra-concentrated calibration standards for specialized research diagnostic methods requiring minimal sample volumes
– University teaching labs— advanced research demonstrations of specialized micro-volume techniques and working with precious materials
– College research groups— preparing concentrated stocks from research compounds available in sub-milligram quantities, standards for specialized micro-analytical techniques
– School science labs — specialized demonstrations of ultra-micro preparation techniques for advanced students
– Industrial QC labs — preparing ultra-concentrated standards from extremely costly or rare reference materials, specialized micro-volume calibration
MANUFACTURING PROCESS:
GWSI manufactures 25 ml ASTM Class A volumetric flasks by melting borosilicate 3.3 glass at 1,650°C and forming the flat-bottom bulb, narrow neck, and precision-bore opening through skilled hand-blowing or precision automated molding. The neck is carefully drawn to exact dimensions ensuring the calibration mark provides Class A accuracy at the 25 ml volume. The glass stopper is precision-ground to match the flask opening for reliable sealing. The entire flask undergoes controlled annealing to eliminate internal stress and ensure dimensional stability.
TESTING PROCESS:
Manual inspection verifies wall thickness uniformity, neck dimensions, stopper fit, and overall geometry on every flask. Automated polarization stress testing then scans the glass for residual strain that could affect dimensional stability. Each Class A flask undergoes NABL gravimetric calibration to verify the exact volume at the calibration mark meets ASTM Class A tolerances, with certification documentation provided for traceability and quality assurance.
PRINTING PROCESS:
The calibration mark, volume designation, temperature specification, and Class A identification are silkscreened onto the flask using ceramic glass inks formulated for borosilicate compatibility. The flasks enter high-temperature kilns where ceramic glass fusing permanently bonds the ink into the glass matrix. This creates durable, legible markings that resist chemical attack, repeated cleaning, and autoclaving—essential for volumetric glassware used in specialized micro-volume work.
PACKAGING PROCESS:
Final sorting removes any flasks with wall defects, neck irregularities, stopper fit issues, or surface blemishes. Approved flasks are individually wrapped with the matched glass stopper secured in place to prevent separation during shipping. They’re packed in heavy-duty double-wall corrugated boxes with shock-absorbing dividers, suitable for domestic distribution or international export to research laboratories requiring specialized micro-volume precision glassware.
MAINTENANCE & SAFETY:
– Clean immediately after use with appropriate solvents or detergents; use specialized micro flask brushes for thorough interior cleaning without scratching
– Autoclave at 121°C / 15 psi with stopper removed; flasks are fully autoclavable and maintain calibration through repeated sterilization
– Handle with extreme care to avoid impacts that could affect calibration accuracy; the compact size requires very precise manipulation during filling
– Store upright with stopper in place to prevent contamination; the 25 ml size is ideal for secure storage in specialized analytical workstations
– Calibrate periodically according to laboratory quality procedures; NABL recalibration services maintain traceability
– Fill to the calibration mark at eye level with the meniscus bottom tangent to the mark for accurate 25 ml volume measurement
*Manufactured by GWSI Glass Lab — Ambala, Haryana, India (The Science City)